Pathak, Ashish Agarwal, Nitin Singh, Poonam Dhaneria, Mamta Prolapse of inverted ileal loops through a patent vitellointestinal duct cholerae O1 strain and its role in hemorrhagic response in RIL model.

Conclusion Our results show the presence of a novel 59-kDa serine protease in a ГЋвЂќhapAГЋвЂќprtV V. RIL assay with culture supernatant of CHA6.8ГЋвЂќprtVГЋвЂќVC1649 strain (FA ratio 0.11+/ГўЛ†вЂ™0.005 nГўв‚¬Е =Гўв‚¬Е 3) and with protease incubated with PMSF and EDTA (FA ratio 0.3+/ГўЛ†вЂ™0.05 nГўв‚¬Е =Гўв‚¬Е 3) induced a significantly reduced FA ratio with almost complete normal villus structure. RIL assay with culture supernatants of strains C6709 (FA ratio 1.1+/ГўЛ†вЂ™0.3 nГўв‚¬Е =Гўв‚¬Е 3), CHA6.8 (FA ratio 1.08+/ГўЛ†вЂ™0.2 nГўв‚¬Е =Гўв‚¬Е 3), CHA6.8ГЋвЂќprtV (FA ratio 1.02+/ГўЛ†вЂ™0.2 nГўв‚¬Е =Гўв‚¬Е 3) and partially purified serine protease from CHA6.8ГЋвЂќprtV (FA ratio 1.2+/ГўЛ†вЂ™0.3 nГўв‚¬Е =Гўв‚¬Е 3) induced fluid accumulation and histopathological studies on rabbit ileum showed destruction of the villus structure with hemorrhage in all layers of the mucosa. The protease activity was partially inhibited by 25 mM PMSF and 10 mM EDTA and completely inhibited by EDTA and PMSF together. The major protein band in native PAGE was identified by MS peptide mapping and sequence analysis showed homology with a 59-kDa trypsin-like serine protease encoded by VC1649. Methodology/Principal Findings We partially purified the residual protease secreted by strain CHA6.8ГЋвЂќprtV from culture supernatant by anion-exchange chromatography. We initiated this study to characterize the protease present in CHA6.8ГЋвЂќprtV strain and study its role in pathogenesis in rabbit ileal loop model (RIL). cholerae O1 strain CHA6.8ГЋвЂќprtV, still retains residual protease activity. Wai, Sun Nyunt Pal, AmitД«ackground Two well-characterized proteases secreted by Vibrio cholerae O1 strains are hemagglutinin protease (HAP) and V. Syngkon, Aurelia Elluri, Sridhar Koley, Hemanta Rompikuntal, Pramod K. Studies on a Novel Serine Protease of a ГЋвЂќhapAГЋвЂќprtV Vibrio cholerae O1 Strain and Its Role in Hemorrhagic Response in the Rabbit Ileal Loop Model (3) The participation of the enterostomal therapist is the preparation of the patient and in the immediate and long-term stomal care has been invaluable and is strongly recommended. There were no postoperative deaths, and a moderate number of complications occurred in 51.8% of the patients.

(2) All loops were performed in a similar manner, most of them placed retroperitoneally, and a vigorous program of postoperative care was followed. Reflux, incontinence, retention, and bladder calculi were additional indications for supravesical urinary diversions. (1) In a six year experience with ileal loops in patients with neurogenic bladder, 49% of the patients were paralyzed, 30% had multiple sclerosis, and 91% had recurrent or persistent urinary tract infection.

Kambouris, A A Allaben, R D Carpenter, W S Shumaker, E J Ileal loop ureteroileostomy in patients with neurogenic bladder.

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